- Cdh1 Mutants
- cdh1∆
- sic1∆ cdh1∆
- sic1∆ cdh1∆ GALL-CDC20
- sic1∆ cdc6∆2-49 cdh1∆
- sic1∆ cdc6∆2-49 cdh1∆ GALL-CDC20
- cdh1∆ cdc6∆2-49
- swi5∆ cdh1∆
- swi5∆ cdh1∆ GAL-SIC1
- cln1∆ cln2∆ cdh1∆
- GAL-CLN2 cln1∆ cln2∆ cdh1∆
- cln1∆ cln2∆ cln3∆ cdh1∆
- GAL-SIC1 cln1∆ cln2∆ cdh1∆
- GAL-SIC1 GAL-CLN2 cln1∆ cln2∆ cdh1∆
- CLB1 clb2∆ cdh1∆
- GAL-CLB2 cdh1∆
- GAL-CLB5 cdh1∆
- cdc14-ts cdh1∆
- cdc15∆ net1-ts cdh1∆
- APC-A cdh1∆
- APC-A cdh1∆ in galactose
- APC-A cdh1∆ GAL-SIC1
- APC-A cdh1∆ GAL-CDC6
- APC-A cdh1∆ multi-copy SIC1
- APC-A cdh1∆ multicopy CDC6
- APC-A cdh1∆ multicopy CDC20
- Cdh1 constitutively active
cdh1∆ cdc6∆2-49
debug: ,
test user =
test db =
Simulation:
Change of parameters: ksf6'=ksf6"=ksf6"'=0, kscdh=0, init CDH1T=CDH1=0.
Length of G1 phase: 60.2 min.
Mass at division: 1.96
Experiments:
Calzada, A., Sacristan, M., Sanchez, E. and Bueno, A. (2001). Cdc6 cooperates with Sic1 and Hct1 to inactivate mitotic cyclin-dependent kinases. Nature 412:355-358.
[Abstract] [Article]
[Abstract] [Article]
Experimental results: Fig. 3b, the mutants are viable and larger. They show a signifant lag in mitosis. Asynchronously growing cells show a marked increase in the percent of cells with 2C DNA contents as compared with WT.
Comments: Problem for the model. The simulation predicts correctly that the mutant cells are viable, but they are smaller than wild-type and have a shorter G2 phase.